NIOS Biology Paper 2024 October Set A
SECTION–A (1 MARK)
1. The bacteria that help in the fixation of atmospheric nitrogen:
(A) Rhizobium
(B) Streptomyces
(C) Salmonella
(D) Lactobacillus
2. Which one of the following represent the gametophyte of fern?
(A) Indusium
(B) Sorus
(C) Prothallus
(D) Frond
3. Which one of the following is the living part of xylem?
(A) Tracheid
(B) Vessel
(C) Xylem Fibre
(D) Xylem Parenchyma
4. Name the cells that are seen in the lymph nodes and attack bacteria
(A) Lymphocytes
(B) Monocytes
(C) Granulocytes
(D) Erythrocytes
5. The blood protein that provides factors for clotting is
(A) Albumin
(B) Fibrinogen
(C) Globulin
(D) Thrombin
6. Identify “A” in the given diagram

(A) Cerebellum
(B) Cerebrum
(C) Medulla oblongata
(D) Pons
7. Which one of the following is not produced due to undersecretion of thyroxine?
(A) Goitre
(B) Cretinism
(C) Myxoedema
(D) Rickets
8. Which one of the following adaptations is seen in plants to promote self-pollination?
(A) Cleistogamy
(B) Dichogamy
(C) Unisexuality
(D) Self-sterility
9. Corpus luteum produces
(A) Oestrogen
(B) Androgens
(C) Progesterone
(D) Prolactin
10. In the following flow chart A, B, C, and D represent

(A) A-AA+ZW, B-A+W, C-AA+ZW, D-AA+ZW
(B) A-AA+ZZ, B-A+W, C-AA+ZW, D-AA+ZW
(C) A-AA+ZW, B-A+Z, C-AA+ZW, D-AA+ZW
(D) A-AA+ZW, B-A+W, C-AA+ZW, D-AA+ZZ
11. The formation of RNA from DNA is known as
(A) Translation
(B) Transcription
(C) Transformation
(D) Transduction
12. In the given food chain identify the trophic level of frog.

(A) First trophic level
(B) Second trophic level
(C) Third trophic level
(D) Tertiary trophic level
13. The biological community in an area or ecosystem is a complex network of interactions. Interactions may be of various types. In commensalism type
(A) One species gets benefitted, and the other species is neither harmed nor benefitted.
(B) One species is inhibited and the other is unaffected.
(C) One species is benefitted, and the other is harmed and inhibited.
(D) Both the species are benefitted.
14. Biotechnology helps in synthesizing enzymes from cloned genes. Your friend wants to
manufacture meat tenderisers. Which enzyme would you recommend to him?
(A) Lipases
(B) Proteases
(C) Amylases
(D) Glucoisomerases
15. Which one of the following is not the step of microinjection process in biotechnology?
(A) Collection and in vitro maturation of oocytes
(B) In vitro fertilization with male semen
(C) Microinjection of “input DNA” into male pronuclei
(D) Infection of pre-implantation embryos with retroviruses carrying foreign DNA
16. Identify the immunoglobulin found in highest concentration in our body
(A) IgD
(B) IgA
(C) IgG
(D) IgM
SECTION – B (2 Marks)
17. Name the following
(i) Excretory organs of earthworm – __________
(ii) Naked seeds are the characteristic feature of this group of plants – __________
Ans:
- (i) Nephridia.
- (ii) Gymnosperms
18. Match Column I with the functions given in Column II.
| Column-I | Column-II |
| (i) Pneumatophores | (a) Roots with chlorophyll and manufacture food |
| (ii) Haustoria | (b) Roots developing from nodes and provide anchorage |
| (c) Roots which grow vertically up in the air and help in respiration | |
| (d) Roots which penetrate the host plant and suck food from host |
Ans:
- (i) Pneumatophores- (c) Roots which grow vertically up in the air and help in respiration
- (ii) Haustoria- (d) Roots which penetrate the host plant and suck food from the host
19. Observe the diagrams given below and assign the correct name from the list supplied. (Attempt any two parts from A-D). (Rhizome, Tuber, Corm, Bulb)

(Rhizome, Tuber, Corm, Bulb)
Ans:
- Rhizome – A
- Tuber – D
- Corm – B
- Bulb – C
20. Photosynthesis occurs in green parts of the plants, mostly the leaves which have chloroplasts. The thylakoids of chloroplast contain pigments which absorb light of different wavelengths and carry out the photochemical reactions. These pigments are packed into functional clusters called photosystems-PS-I and PS-II.
(a) Name the pigments that constitute the photosystems.
(b) What is the function of Photosystems?
Ans:
- (i) Chlorophyll a, P700, P680.
- (ii) To trap the solar energy and convert it into chemical energy
21. Study the flow chart and complete it by substituting A-D with suitable words. (Attempt any two parts from A-D).

Ans:
- A – Glycolysis
- B – Ethanol + CO2
- C – Anaerobic respiration in Muscle cells
- D – Krebs’ Cycle
22. Supply the technical term for the following
(a) Division of other cells in embryo sac like synergids or antipodal cells to give rise to additional embryos.
(b) The process in which the zygote may divide to give rise to two or more cells each of which develops into a separate embryo.
Ans:
- Adventive polyembryony.
- Cleavage polyembryony.
23. Match the statement given in Column I with the right option in Column II
| Column-I | Column-II |
| (i) Copper-T | (a) Interfere with the ovulation process and prevent fertilization |
| (ii) Condoms | (b) Prevent sperm from meeting the ovulated egg |
| (c) Implantation is not possible | |
| (d) Permanently prevent fertilization |
Ans:
(i) Copper-T – (a) Interfere with the ovulation process and prevent fertilization
(ii) Condoms – (b) Prevent sperm from meeting the ovulated egg
24. Read the following passage and fill in the blanks with appropriate words.
(Attempt any two parts A-D)
Criss Cross inheritance in humans
A .and B . are examples of sex-linked inheritance in humans. The defective gene is located on C . chromosome. Thus a single defective gene causes disease in males while two defective genes ( D . condition) only can cause the disease in females.
Ans:
- A – Red-green colour blindness
- B – Haemophilia
- C – X chromosome
- D – Homozygous condition
25. Aquatic ecosystems are classified on the basis of salinity into two types-Fresh water and Marine. The common flora and fauna found in fresh water bodies like rivers, lake include phytoplanktons, water hyacinth, water lily and zooplankton, crab, and fishes, respectively. Wetlands are between aquatic and terrestrial ecosystems. They show an edge effect and form an ecotone.
(a) What is the study of freshwater ecosystem known as?
(b) Define ecotone.
Ans:
- Limnology
- Ecotone is a transitional zone between two ecosystems.
26. Study the diagram given below carefully and answer the questions that follow. Identify the processes “A” and “B”.

Ans:
- A – Photosynthesis
- B – Respiration
27. Given below is the list of vitamins with their functions. Choose the incorrect pairs of vitamins with reference to their function.
(a) Vitamin A: Maintenance of vision
(b) Vitamin C: Formation of blood
(c) Vitamin D: Healthy bones and teeth
(d) Vitamin K: antioxidant: ageing vitamin
Ans: Incorrect pairs are,
- (b) Vitamin C: Formation of blood
- (d) Vitamin K: antioxidant: ageing vitamin
28. Fill in the spaces A and B in the sentences given below
(a) Cell-mediated immune response is mediated by A.
(b) Humoral immune responses is mediated by B.
Ans:
- Cytotoxic T-cells and natural killer cells.
- Antibodies.
SECTION – C (2 Marks)
29. What are the four nutritional categories found in bacteria?
Ans: The four nutritional categories found in bacteria are,
- Autotrophs
- Saprotrophs
- Symbionts
- Parasites
30. Analyse the differences between striated muscles and unstriated muscles with reference to their shape.
Ans:
| Striated muscles | Unstriated muscles |
| Elongated, cylindrical, and unbranched fibres | Spindle-shaped and tapering. |
| Myofibrils are so arranged in the cytoplasm that striations are seen. | No such striations are visible here, as the myofibrils are not uniformly arranged. |
31. (a) Name the two biochemical reactions for biosynthesis of amino acids in plants.
Ans:
(i) Reductive amination reaction
(ii) Transamination reaction
OR
(b) Name the two proteins found in root nodules and help in nitrogen fixation.
Ans:
(i) Leghaemoglobin
(ii) Nodulins
32. Identify A in the diagram given below and state its function

32. Identify A in the diagram given below and state its function
Ans:
- A- Sinu auricular node.
- It initiates and regularises the heartbeat. Therefore, it is also called the pacemaker.
33. Identify the type of neuron given in the diagram and state the function of axon.

Ans:
- It is a neuron that conducts nerve impulses.
- Axon: An axon carries the impulse from the cell body towards its terminal branches, which may either pass on the impulse to another neuron, or into a muscle or gland to bring about the required action.
34. (a) Why is the process of fertilization in flowering plants said to be double fertilization?
Ans: In flowering plants, one sperm fuses with the egg (syngamy) and forms a diploid zygote. The other sperm fuses with the secondary nucleus to form the primary endosperm nucleus, which is triploid in nature. Since two types of fusion, syngamy and triple fusion, take place in an embryo sac, the process is termed double fertilization.
OR
(b) What is the function of Endosperm in the flowering plants? Write the names of any
types of endosperms.
Ans: Endosperm provides food to the developing embryo. Types of endosperm are nuclear type, cellular type, and helobial type.
35. Observe the given diagram and answer the questions that follow.

35. Observe the given diagram and answer the questions that follow.
(a) In which direction does the enzyme DNA polymerase catalyze DNA replication – 5′ to 3′ or 3′ to 5′?
(b) Which enzymes join the Okazaki fragments to form a complete DNA strand?
Ans:
- In 5’-3’ direction
- Enzyme ligase
36. A group of food items is given in the table. Categorize them into energy-providing foods and bodybuilding foods. Cereals, legumes, meat, sugar, oil, fish, egg, jaggery
Ans:
- Energy-providing foods: Cereals, Sugar, oil, and jaggery
- Body Building Foods: Legumes, meat, fish, and eggs.
37. (a) Following is the representation of how a plasmid isolated from bacterium that causes galls in several plants is used to produce transgenic plants.

(i) Name the bacterium that causes galls in plants.
(ii) Name the plasmid used to transmit the desired genes.
Ans:
(i) Agrobacterium tumefaciens causes galls (tumours) in plants.
(ii) Ti plasmid
OR
(b) Given below is the picture of a normal mouse (A) and a transgenic mouse (B).

Why is there an increase in the size of mouse (B)?
Ans: A Gene for growth hormone from rats was microinjected into mouse eggs. These mice grew larger than their littermates. This was because the rat gene got integrated into the mouse DNA and was being expressed.
SECTION – D (3 Marks)
38. (a) Define obesity.
(b) List any two causes of obesity.
(c) State two Harmful effects of obesity.
Ans:
- The overweight and bulkiness of a person’s body due to the accumulation of carbohydrates and fats is called obesity.
- Two causes of obesity are overeating and insufficient exercise
- Hypertension and atherosclerosis are two harmful effects of obesity.
39. (I) Illustrate the 3 major phases in glycolysis.
Ans: The three major phases of glycolysis are activation, splitting of glucose, and oxidation.

OR
(II) Distinguish between C3 and C4 plants with reference to the following characteristics.
(a) Carbon dioxide acceptor
(b) Carbon dioxide fixing enzymes
(c) First product of photosynthesis
Ans: Features C3 Plants C4 Plants
| C3 Plants | C4 Plants | |
| (a) Carbon dioxide acceptor | 5-Carbon sugar- ribulose biphosphate or RuBP | Phosphoenol pyruvic acid or PEP, |
| (b) Carbon dioxide fixing enzymes | Ribulose bisphosphate carboxylase/oxygenase or Rubisco. | Phosphoenol pyruvate carboxylase (PEP carboxylase) |
| (c) First product of photosynthesis | 3-Phosphoglyceric acid (PGA). | Oxaloacetic acid (OAA). |
40. (I) Observe the Lac operon diagram given below and answer the questions that follow.

(a) Which substance acts as inducer in this operon?
(b) Where does RNA polymerase bind to initiate transcription?
(c) What are the genes z, y, a called as?
Ans:
- Lactose is the inducer in this operon.
- RNA polymerase binds to the promoter gene p.
- Genes z, y, and a are structural genes, where gene a codes for the enzymes galactosidase, permease, and transacetylase.
OR
(II) Study the karyotype given below and answer the questions that follow.

(II) Study the karyotype given below and answer the questions that follow.
(a) What is this disorder known as?
(b) How many chromosomes are present in the individuals with this disorder?
(c) State one typical feature of this syndrome.
Ans:
- Klinefelter’s syndrome
- This individual male has 47 chromosomes with an extra X chromosome.
- A typical feature of this syndrome is being tall and mentally retarded.
41. Deficiency in minerals in our food may lead to deficiency diseases. Name which mineral deficiency may lead to the following :
(a) Anaemia
(b) Osteomalacia
(c) Goitre
Ans:
- Deficiency of Vitamin B12
- Deficiency of Vitamin D
- Iodine deficiency
SECTION – E (5 Marks)
42. (a) A snapdragon plant with red flowers (RR) is crossed with a plant with white flowers (rr). Work out the cross up to F2 generation. Find out the phenotypic and genotypic ratio.
Ans:
| Parents | RR x rr |
| Gametes | R, R x r, r |
| F1 | Rr (Pink) |
| F2 | 1 Red: 2 Pink: 1 White |
| 1RR: 2Rr: 1rr |
OR
(b) A tall pea plant with red flowers (TTRR) is crossed with white dwarf plants (ttrr). Work out the phenotypic ratio in F2 for this cross.
Ans:
Cross between TTRR (tall red) x ttrr (Dwarf white)
Gametes formed by TTRR are TR, TR
Gametes formed by ttrr are tr and tr
| Gametes | TR | TR |
| tr | TtRr – Tall red | TtRr – Tall red |
| tr | TtRr – Tall red | TtRr – Tall red |
The F1 generation is all Tall red with a genotype of TtRr.
F2 is crossed between TtRr x TtRr
The gametes procured are TR, Tr, tR, tr
| Gametes | TR | Tr | tR | tr |
| TR | TTRRTall red | TTRrTall red | TtRRTall red | TtRrTall red |
| Tr | TTRrTall red | TTrrTall white | TtRrTall red | TtrrTall white |
| tR | TtRRTall red | TtRrTall red | ttRRDwarf red | ttRrDwarf red |
| tr | TtRrTall red | TtrrTall white | ttRrDwarf red | TtrrDwarf white |
The phenotypic ratio in F2 is 9 tall red: 3Dwarf red: 3 Tall white: 1 Dwarf white
43. (a) What is Chemosynthesis? Write any four differences between chemosynthesis and photosynthesis.
Ans: Chemosynthesis is the process in bacteria where they use chemical energy to reduce CO2 for carbohydrate synthesis.
| Chemosynthesis | Photosynthesis |
| It occurs only in colourless anaerobic bacteria | This process occurs in all green plants, including green bacteria. |
| During this process, CO2 is reduced to carbohydrates without light and chlorophyll. | CO2 and H2O are converted into carbohydrates in the presence of light and chlorophyll. |
| Here, chemical energy released during the oxidation of inorganic substances is used up to synthesise carbohydrates. | Light energy is converted into chemical energy and stored in the form of carbohydrates. |
| No pigment molecule is involved, and oxygen is not evolved. | Several pigments are involved, and oxygen is evolved as a by-product. |
| No photophosphorylation takes place. | Photophosphorylation takes place, i.e., ATP is produced. |
OR
(b) With the help of a diagram explain the external structure of human heart.
Ans:

Additional Study Materials
- NIOS Class 12 Biology PYQ April 2025 Set A
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